Research

October 31, 2012

Genetic diversity of the endangered and medically important Lycium ruthenicum Murr. revealed by sequence-related amplified polymorphism (SRAP) markers

Sequence-related amplified polymorphism (SRAP) markers were used to assess the genetic diversity and population genetic structure in fourteen wild populations of Lycium ruthenicum from Northwestern China. Thirty-one selected primer combinations produced 468 discernible bands, with 398 (85.04%) being polymorphic, indicating relatively high genetic diversity at the species level. Analysis of molecular variance showed that the genetic variation was found mainly within populations (84.45%), but variance among populations was only 15.55%. And there was a moderate genetic differentiation (Gst  0.2155) among populations. Mantel test revealed a significant correlation between genetic and geographic distances (r  0.303, P  0.004), and the unweighted pair-group method using arithmetic average clustering and principal coordinates analysis demonstrated similar results. A total of nine significant (P < 0.05) correlations were detected between three indices of genetic diversity and seven ecogeographic factors. Also recommendations for conservation of the endangered species resources and breeding program are proposed.

Fig. 1. Geographic distribution of the 14 sampled populations of L. ruthenicum in northwest China.
 

Additional Information:
1. Author Information: Zenggen Liu, Qingyan Shu, Lei Wang, Minfeng Yu, Yanping Hu, Huaigang Zhang, Yanduo Tao, Yun Shao
   Correspondence: zangyaokaifa@163.com
2. Published : Biochemical Systematics and Ecology 45 (2012) 86–97

 

Genetic diversity of the endangered and medically important Lycium ruthenicum Murr. revealed by sequence-related amplified polymorphism (SRAP) markers