In this study, 53 actinomycetes strains were isolated from desert ecosystems located in northeast of Qinghai-Tibet Plateau and grouped into four RFLP patterns. Twenty-six actinomycetes with obvious morphology differences were chosen for phylogenetic diversity study and antimicrobial testing. As a result, the 16S rRNA gene sequencing showed that these strains belonged to Streptomyces, Micromonospora, Saccharothrix, Streptosporangium and Cellulomonas, and that most of the strains had antimicrobial bioactivity. The PKS and NRPS genes detection indicated diversified potential bioactive products of actinomycetes in this ecosystem. Among these strains, Sd-31 was chosen to study the bioactive products using HPLC-MS because of its optimum antimicrobial bioactivity. The result showed that it might produce Granatomycin A, Granatomycin C, and an unknown compound.
Fig. 16S rRNA-RFLP patterns of isolates from a desert ecosystem in the northeast of the Qinghai-Tibet plateau
Additional Information:
1. Author Information: Dong Ding, Guichen Chen, Bochan Wang, Qilan Wang, Demei Liu, Min Peng & Ping Shi
Correspondence: pengm@nwipb.ac.cn
2. Published : Ann Microbiol DOI 10.1007/s13213-012-0469-9