A simple and mild method for the determination of fatty acids (C1 – C10) based on a
condensation reaction using 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) as labeling
reagent with capillary zone electrophoresis has been developed. The detection was performed
with a diode array detector at 254 nm. A 58.5 cm 9 50 lm i.d. (50 cm effective length)
untreated fused-silica capillary was used. To optimize the separation conditions, the background
electrolyte concentration, column temperature, voltage and other factors were evaluated.
The optimal separation conditions were as follows: 30 mmol L-1 borate buffer
(pH 9.5), 15 mmol L-1 b-CD, temperature at 20 C, pressure 50 mbar and injection time 8 s.
Under the established conditions, 10 fatty acid derivatives could be well-separated within
17 min. The linearity was in the range of 0.07–5.0 lmol L-1. Detection limits (at a signalto-
noise ratio of 3) were in the range of 0.027–0.042 lmol L-1. The fatty acids from the
extracted Funaria Hedw. and Selaginella samples were determined with satisfactory results.
condensation reaction using 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) as labeling
reagent with capillary zone electrophoresis has been developed. The detection was performed
with a diode array detector at 254 nm. A 58.5 cm 9 50 lm i.d. (50 cm effective length)
untreated fused-silica capillary was used. To optimize the separation conditions, the background
electrolyte concentration, column temperature, voltage and other factors were evaluated.
The optimal separation conditions were as follows: 30 mmol L-1 borate buffer
(pH 9.5), 15 mmol L-1 b-CD, temperature at 20 C, pressure 50 mbar and injection time 8 s.
Under the established conditions, 10 fatty acid derivatives could be well-separated within
17 min. The linearity was in the range of 0.07–5.0 lmol L-1. Detection limits (at a signalto-
noise ratio of 3) were in the range of 0.027–0.042 lmol L-1. The fatty acids from the
extracted Funaria Hedw. and Selaginella samples were determined with satisfactory results.
The research has been published on Chromatographia 2010, 71, June (No. 11/12) 1125–1129
Xin-Wei Bai1, Cui-Hua Song1, Jin-Mao You1,2,&, Zhi-Wei Sun1,2, Yan-Yan Fu1, Guo-Liang Li2