Research

 A rapid and sensitive method was optimized
and validated for the separation and quantification of
derivatized monosaccharides in polysaccharide from
Potentilla anserine L. using 1-naphthyl-3-methyl-5-
pyrazolone (NMP) as precolumn derivatization reagent by
capillary zone electrophoresis (CZE). On the basis of the
optimum conditions, nine NMP-derivatized monosaccharides
achieve baseline resolution within 16 min. The
developed method has been successfully applied to analyze
component monosaccharides of three Potentilla anserine L.
samples, which were obtained by gradational precipitation
with 50, 70, and 90% aqueous ethanol, respectively. The
polysaccharide precipitated from 50% ethanol solvent was
composed of fucose, mannose, xylose, glucuronic acid,
glucose, rhamnose, galacturonic acid, galactose, arabinose
in molar proportion of 1:1.65:1.99:5.08:7.38:8.14:13.05:
27.41:39.02; the corresponding molar proportions for
polysaccharide obtained from 70% ethanol solvent were
1:1.64:1.65:4.52:13.96:9.13:26.30:10.52:18.00; fucose and
galacturonic acid were not found in the polysaccharide
precipitated from 90% ethanol solvent, and mannose,
xylose, glucuronic acid, glucose, rhamnose, galactose and
arabinose were determined with molar proportion of
1:0.87:1.77:2.78:1.69:2.58:2.49. Quantitative recoveries of
the component monosaccharides in the polysaccharide
were in the range of 93.3–105.1% and the relative standard
deviation (RSD) values fell within 3.4–6.3%, respectively.
The results demonstrated that the proposed CZE method
was precise and sensitive for the analysis of the composition
of polysaccharide.