Suo Yourui and his team established a method of using high-speed counter-current chromatography (HSCCC) for preparative isolation and purification of oligostilbenes from the ethanol extracts of seed kernel of Iris lactea Pall. var. chinensis (Fisch.) Koidz.
They separated and purified four oligostilbenes by HSCCC with two sets of two-phase solvent system, n-hexane-ethyl acetate-methanol-water (3:6:4.2:5.5, v/v/v/v) in the head-to-tail elution mode for the first separation to mainly isolate vitisin A (58 mg), ε-viniferin (76 mg) and peak II (43 mg) from 300 mg of the crude ethanol extracts, and then light petroleum–ethyl acetate–methanol–water (5:5:3:6, v/v/v/v) in the tail-to-head elution mode for the second separation to isolate vitisin B (52 mg) and vitisin C (11 mg) from 100 mg of peak II.
The purities of the isolated four oligostilbenes were all over 95.0% as determined by HPLC. Vitisin A, vitisin B and vitisin C, resveratrol tetramers, were isolated from Iris lactea for the first time.
The preparation of crude samplewas simple and the HSCCC method for the isolation and purification of four oligostilbenes was rapid, efficient and economical.
Fig. The photographs of (1) the flowers, (2) seeds, and (3) seed kernels of Iris lactea.
1 Author Information: Huanhuan Lv, Honglun Wang, Yanfeng He, Chenxu Ding, Xiaoyan Wang,Yourui Suo.
Correspondence: firstname.lastname@example.org (H. Wang).
2 Published: Journal of Chromatography B, 988 (2015) 127–134.