WANG Honglun and his teams isolated and purified flavonoids and polyhydroxystilbenes from Trigonella foenum-graecum L. seed extracts using high-speed counter-current chromatography (HSCCC) in two steps with two different kinds of solvent systems.
In the first step, n-hexane:ethyl acetate:methanol:water (1:5:1:5, v/v) was used as the twophase solvent system, desoxy-rhaponticin and rhapontigenin were purified, and orientin, vitexin, isovitexin, and rhaponticin were eluted together.
In the second step, ethyl acetate:water (1:1, v/v) was used as the two-phase solvent system, and orientin, vitexin, rhaponticin, and isovitexin were isolated and purified. Purities of the isolated compounds were determined using HPLC, while UV spectra, 1H-NMR, and 13C-NMR aided in structure identification.
FIG. HPLC chromatograms of Trigonella foenum-graecum L. samples and HSCCC peak fractions. HPLC chromatograms for sample 1 (a) and sample 2 (b).
1 Author Information: Yanfeng He, Huanhuan Lv, Xiaoyan Wang, Yourui Suo,and Honglun Wang
2 Published: Separation Science and Technology,2014, 49: 580–587