The response surface methodology was employed to optimize the integrated extraction parameters of cordycepic acid and cordycepin from cultured Cordyceps militaris (L.) link based on a single-factor experiment.
The Box-Behnken design with three independent variables i.e., microwave power (W), water/material ratio (mL/g) and extraction time (min) was used.
The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis.
The three-dimensional response surface plot and contour plot derived from the mathematical models were applied to determine the optimal conditions.
The optimum extraction condition was obtained as follows: microwave-assisted extraction, microwave power of 649.33 W, extraction time of 5.74 min, water/material ratio of 38. 99 mL/g and extraction number of three.
The yield of cordycepic acid and cordycepin were 2.47 and 0.79 %, respectively.
Under these conditions, the experimental values of 3.12 and 0.75 % well agreed with those predicted by the model.
Additional Information:
1 Author Information: Li Deng, Tong-Yong Zhou, Li Pi, Xiao-Hui Zhao, Tao Han, Yi-Kang Li and Fa Han
Correspondence: designdl@163.com.
2 Published: Asian Journal of Chemistry,2013,25(14)