Research

October 31, 2011

Molecular tagging of a stripe rust resistance gene in Aegilops tauschii

Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most important diseases of common wheat (Triticum aestivum L.). China has the largest stripe rust epidemic areas in the world and yield losses can be large. Aegilops tauschii Coss, the D-genome progenitor of common wheat, includes two subspecies, tauschii and strangulata (Eig) Tzvel. The ssp. strangulata accession AS2388 is highly resistant to the prevailing physiological races of PST in China, and possesses a single dominant gene for stripe rust resistance. In order to tag this gene, AS2388 was crossed with the highly susceptible ssp. tauschii accession AS87. The parents, F2 plants, and F2:3 families were tested at adult plant stage in field trials with six currently prevailing races. Simple sequence repeat (SSR) primers
were used to identify molecular markers linked to the resistance gene. SSR markers Xwmc285 and Xwmc617 were linked to the resistance gene on chromosome arm 4DS flanking it at 1.7 and 34.6 cM, respectively. Based on the chromosomal location, this gene temporarily designated as YrAS2388 is probably novel. The resistance in Ae. tauschii AS2388 was partially expressed in two newly developed synthetic hexaploid backgrounds.

Additional Information:
1. Author Information:Lin Huang, Lian-Quan Zhang, Bao-Long Liu, Ze-Hong Yan, Bo Zhang, Huai-Gang Zhang, You-Liang Zheng and Deng-Cai Liu
Correspondence:  hgzhang@nwipb.ac.cn, dcliu7@yahoo.com.
2. Published : Euphytica (2011) 179:313–318

Molecular tagging of a stripe rust resistance gene in Aegilops tauschii .pdf