Supercritical CO2 fluid extraction (SFE-CO2)
of Zanthoxylum bungeanum (Z. bungeanum) seed oil was
investigated. To optimize the SFE process, three-level
Box-Behnken factorial design and response surface methodology
(RSM) were applied to optimize the extraction
conditions, including pressure, temperature and amount of
modifier. The optimum conditions were as follows:
extraction pressure, 29.28 MPa; extraction temperature,
41.19 C; and the added amount of modifier, 10.94%. The
experimental results showed that the maximum extraction
yield was 21.85 ± 0.23% (n = 3) under the proposed
conditions. The compositional analysis of Z. bungeanum
seed oil was performed by HPLC-FLD-MS using a new
labeling reagent of 2-(11H-benzo[a]carbazol-11-yl)-ethyl-
4-methyl benzenesulfonate (BCETS). The results indicated
that the Z. bungeanum seed oil contained mainly unsaturated
fatty acids, including C18:3, C22:6, C20:4, C18:2,
C18:1 and C20:1, which accounted for 84.0% (mass percentage)
of the total amount. The antioxidant activity of
seed oil obtained by Box-Behnken design concerning the
DPPH radical was investigated, and this indicated that the
pressure and the amount of added modifier had positive
effects on the antioxidant activity, but the effect of the
temperature elevation is complicated, depending on the
nature of the extracted contents.
The research has been published on J Am Oil Chem Soc
DOI 10.1007/s11746-010-1644-4
Lian Xia • Jinmao You • Guoliang Li •
Zhiwei Sun • Yourui Suo